Measurement of nasal potential difference in mild asthmatics.

نویسندگان

  • Nancy C Chung
  • Beate Illek
  • Jonathan H Widdicombe
  • Horst Fischer
چکیده

STUDY OBJECTIVE To determine whether ion transport or barrier function across the nasal epithelium are altered in asthmatics. DESIGN In this pilot study, the nasal potential difference (PD) was measured using the technique established by Knowles and colleagues. A flowing agar bridge made electrical contact with the surface of the nasal epithelium along the floor of the nose. This bridge was referenced to a cutaneous electrode to determine the PD across the nasal epithelium. Changes in nasal PD in response to amiloride, chloride-free medium, and chloride-free medium containing isoproterenol were measured, and responses of asthmatics and healthy control subjects were compared. PARTICIPANTS Measurements were made in eight adult nonasthmatic subjects and 6 adult asthmatic subjects. All asthmatics had mild intermittent asthma. MEASUREMENTS AND RESULTS Continuous measurements of nasal PD were obtained while the nasal surface was perfused consecutively with saline solution (NaCl-containing solution), saline solution plus 100 micro mol/L amiloride, chloride-free solution plus amiloride, and chloride-free solution with amiloride plus 10 micro mol/L isoproterenol (a beta-adrenergic agonist). No significant differences in baseline PD or change in PD in response to changes in perfusate were found between the two groups. CONCLUSIONS Our results suggest that ion transport and barrier function of patients with mild asthma are normal. Therefore, by contrast to cystic fibrosis, changes in salt and water transport across airway epithelium may not contribute to accumulation of mucous secretions in asthma.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Different Gene Expressions of Alpha and Beta Glucocorticoid Receptors in Asthmatics

The response to glucocorticoids (GCs) therapy classifies severe refractory asthma (SRA) and mild asthma, so the glucocorticoid receptors (GCRs) gene expression may be involved in SRA pathogenesis. Thus, it is aimed to compare the expression levels of two GCR isoforms (GCRα and GCRβ) in SRA, mild asthmatics, and healthy controls. Total RNA was isolated from the peripheral blood mononuclear lymph...

متن کامل

Initiating oral breathing in response to nasal loading: asthmatics versus healthy subjects.

Factors influencing nasal versus oral breathing in asthmatics are not well understood. The current authors hypothesised that asthmatic subjects have enhanced perception of nasal threshold loads, and switch from nasal to oral breathing at a lower load than healthy subjects. In total, 15 mild asthmatic and 20 healthy control subjects breathed nasally via an inspiratory threshold loading device. N...

متن کامل

Different Gene Expressions of Alpha and Beta Glucocorticoid Receptors in Asthmatics

The response to glucocorticoids (GCs) therapy classifies severe refractory asthma (SRA) and mild asthma, so the glucocorticoid receptors (GCRs) gene expression may be involved in SRA pathogenesis. Thus, it is aimed to compare the expression levels of two GCR isoforms (GCRα and GCRβ) in SRA, mild asthmatics, and healthy controls. Total RNA was isolated from the peripheral blood mononuclear lymph...

متن کامل

Pale nasal mucosa affects airflow limitations in upper and lower airways in asthmatic children

BACKGROUND Severe asthmatics are thought to have severer rhinitis than mild asthmatics. A pale nasal mucosa is a typical clinical finding in subjects with severe allergic rhinitis. OBJECTIVE The aim of this study was to investigate whether a pale nasal mucosa affects airflow limitations in the upper and lower airways in asthmatic children. METHODS Rhinomanometry, nasal scraping, and spirome...

متن کامل

Level of Fatty Acid Binding Protein 5 (FABP5) Is Increased in Sputum of Allergic Asthmatics and Links to Airway Remodeling and Inflammation

BACKGROUND The inflammatory processes in the upper and lower airways in allergic rhinitis and asthma are similar. Induced sputum and nasal lavage fluid provide a non-invasive way to examine proteins involved in airway inflammation in these conditions. OBJECTIVES We conducted proteomic analyses of sputum and nasal lavage fluid samples to reveal differences in protein abundances and composition...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Chest

دوره 123 5  شماره 

صفحات  -

تاریخ انتشار 2003